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The bupropion uk nhs flaccidly first fusion protein comprises a polypeptide which binds to a tet operator sequence in the presence of tetracycline or a tetracycline analogue, operatively linked to a polypeptide which activates transcription in eukaryotic cells (eg, a transactivator fusion protein of the invention, such as a mutated Tn10-derived Tet repressor linked to a VP16 activation region).
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In another embodiment, the present invention is directed toward a modified TetR(B) protein comprising a plurality of amino acid substitutions at positions selected from the group consisting of positions 59, 70, 71, 91, 95, 96, 98, 99, 100, 101, 103, 110, 114, 127, 157, 158, 159, 160, 178, 188, 192, 194, 196, 200, and 205 of the TetR(B) protein as depicted in SEQ ID NO: 36, wherein said modified TetR(B) protein binds a TetR(B) operator sequence with greater affinity in the presence of tetracycline than in the absence of tetracycline?
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